FastQCFastQC Report
Sun 26 Apr 2020
432_S75_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename432_S75_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6031981
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT820641.3604817389179442No Hit
CACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTG631121.0462897678225445No Hit
GCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGACTG568910.9431561538406703No Hit
CTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGAC235790.3908997723964979No Hit
TGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGACT162420.26926477387776915No Hit
TTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAA150490.24948686012107799No Hit
GTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGACTGAGTT145370.2409987697242415No Hit
ACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGA127810.21188727219134143No Hit
GTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGA126130.20910211752987948No Hit
CGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGAG124230.20595224023417846No Hit
TCGCTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT122330.20280236293847742No Hit
CCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGA119870.1987241007556224No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGAGC118590.1966020781564133No Hit
GACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGACTGAGTTGCTTC117320.1944966338587605No Hit
CCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGACTGA113340.18789846983934466No Hit
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA111240.1844170265125172No Hit
TCGGGTTTATTAATTTTCTTAAAATTTGTTCTTCGTAGAGAAAAGTTGGA110580.1833228586098No Hit
TCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAG103130.1709720239503407No Hit
GGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGG101490.16825318249510401No Hit
GGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG100920.1673082193063937No Hit
TTGCTGCAGTTAAAAAGCTCGTAGCTGGATCTCGGGTCCAGGCTGGCGGT99870.16556749764297998No Hit
TGGCCGAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGAAT97800.16213578922082147No Hit
GTCGGGTTTATTAATTTTCTTAAAATTTGTTCTTCGTAGAGAAAAGTTGG97000.1608095250963158No Hit
TTTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAAT96190.15946668267025377No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG89780.1488399913726519No Hit
CGGGTCCAGGCTGGCGGTCCGCCTCGTTGCGGTCACTGCCTGTCCTGACC88830.14726505272480137No Hit
GGTCGGGTTTATTAATTTTCTTAAAATTTGTTCTTCGTAGAGAAAAGTTG85990.14255681508280613No Hit
GGTTAGAGGTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGC84860.14068346700694184No Hit
CGCTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTG79710.1321456417054364No Hit
GGCCGAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGAATC74580.12364097300704362No Hit
CAGACGGCCTACAGCGAAAGCAATTGCCAAGCATGTTTTCATTAGTCAAG74290.12316020226191031No Hit
TTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATT71290.11818671179501396No Hit
TTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTG70450.11679413446428297No Hit
TGCTGCAGTTAAAAAGCTCGTAGCTGGATCTCGGGTCCAGGCTGGCGGTC69760.1156502316568968No Hit
GGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGACTAAGCCATGCA68450.1134784741530187No Hit
GCTGCAGTTAAAAAGCTCGTAGCTGGATCTCGGGTCCAGGCTGGCGGTCC68290.11321322132811758No Hit
GTTTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA67330.11162170437871075No Hit
TGGATCCTCGCCAGACGGCCTACAGCGAAAGCAATTGCCAAGCATGTTTT66480.11021254874642343No Hit
TGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCAATTGCCAA62980.10441014320171102No Hit
TGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGAC61650.10220522909472028No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGAGCA60410.10014951970173647No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)