FastQCFastQC Report
Sun 26 Apr 2020
431b_S8_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename431b_S8_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6371191
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA284290.44621170515842334No Hit
CCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAA175860.2760237450109406No Hit
CAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAAGGGCAGG78680.12349339393529404No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT73760.1157711329012111No Hit
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG73730.1157240459436862No Hit
TCACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT66600.10453304570526922No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA65000.10202174130394144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)