FastQCFastQC Report
Sun 26 Apr 2020
41_S62_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename41_S62_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8285971
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA180030.2172708545564545No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG153910.18574769329991622No Hit
GGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGACTAAGCCATGCA139840.16876718492014997No Hit
TCACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT137090.1654483222304302No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT117120.1413473448072652No Hit
TCAATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCG102560.1237754754391489No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT100980.12186863796650989No Hit
CACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTG99930.12060143584861689No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT92410.1115258549661832No Hit
TGGATCCTCGCCAGACGGCCTACAGCGAAAGCAATTGCCAAGCATGTTTT89600.10813458072686954No Hit
CAATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGG89500.10801389481087974No Hit
CGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGT83800.10113479759946058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)