FastQCFastQC Report
Sun 26 Apr 2020
414_S49_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename414_S49_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5575142
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG163880.2939476698530728No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA134340.24096247234599585No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA130980.234935720022916No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT101920.1828114871334219No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA89970.16137705550818257No Hit
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA83350.14950291849068598No Hit
TCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAAAA81840.14679446729787332No Hit
GTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAAA81790.14670478348354177No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT66950.12008662738993914No Hit
AAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTG62340.11181777970857067No Hit
AAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGA59260.10629325674574747No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA56190.10078667054579059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)