FastQCFastQC Report
Sun 26 Apr 2020
412_S74_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename412_S74_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7052231
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA2284513.239414590928743No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG1998862.8343654653399755No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG927541.3152433605762488No Hit
TCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGG774021.097553384170201No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA702290.9958408906344673No Hit
TGTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCC406700.5766969346296229No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT394090.5588160682768333No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT356860.5060242638109841No Hit
CGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGT352540.499898542744842No Hit
GTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGAC215170.3051091207874501No Hit
TACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCT213260.3024007580012623No Hit
TCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACC211640.300103612601459No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT206810.2932547161316752No Hit
CGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGT186510.2644694990847577No Hit
CAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG186210.26404410178849785No Hit
AGTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCC184400.26147753810106333No Hit
GTGAGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA170330.2415264049064757No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT157310.22306416224879758No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT155880.22103643513662558No Hit
CGTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCC151550.21489653416060817No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA150580.2135210829027013No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG141230.20026286716926886No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA140700.19951133194587642No Hit
GGTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA136160.19307365286247713No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAGTTGAATCTCGTATGCCGT133100.1887346004406265TruSeq Adapter, Index 8 (98% over 50bp)
TTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTT128220.18181480442146605No Hit
TGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAA121070.17167616886060597No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA119980.1701305586841951No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC114820.16281372518852544No Hit
CGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACCG112270.1591978481703166No Hit
TCTTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAA108650.15406472079544759No Hit
TAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG108080.15325646593255382No Hit
GGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG106920.15161159638701568No Hit
AGGGCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG106390.15086006116362324No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTGAATCTCGTATGCCGTC101370.14374174640620818TruSeq Adapter, Index 8 (98% over 50bp)
GCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTA99550.14116100280889834No Hit
AAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG99420.14097666398051908No Hit
TTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAA98440.13958703281273685No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA97660.1384809998424612No Hit
CTTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAA97310.1379847029968247No Hit
CCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAA95150.13492184246375366No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAGTTGAATCTCGTATGCCGG94210.13358893093547278TruSeq Adapter, Index 8 (97% over 49bp)
TGAGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG93900.13314935372933756No Hit
ACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATC93080.13198660111956062No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA91570.12984543472838594No Hit
TGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG91020.12906553968524287No Hit
TTGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAGCT90530.12837072410135175No Hit
GGGCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA89110.12635717689905507No Hit
ACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTA86980.12333685609561003No Hit
GATTGGCACCTCGATGTTGAATCAGGGATGATACCTTTAAGGCGTAGAAG86830.12312415744748009No Hit
GCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAA86320.12240098204383833No Hit
GCGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT85410.12111061024518341No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT85020.12055759376004557No Hit
CTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGT83220.11800520998248638No Hit
CAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTAC82680.11723949484921863No Hit
AGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT82050.11634616052707292No Hit
CTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTC80800.11457367179265683No Hit
GCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATA77650.11010700018192823No Hit
GTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG77530.1099368412634243No Hit
ATGTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGC77190.10945472432766312No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG76030.10780985478212497No Hit
TTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAG75910.10763969586362102No Hit
CATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAG73160.10374022064790561No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT73030.10355588181952632No Hit
TACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTT72110.10225133011099607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)