FastQCFastQC Report
Sun 26 Apr 2020
404_S42_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename404_S42_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7000213
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT142460.20350809325373384No Hit
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG135390.193408400572954No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG110450.15778091323792576No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT107780.1539667435833738No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA106780.15253821562286748No Hit
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA98500.14071000410987494No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA88180.12596759555744946No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA87370.12481048790943933No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT85320.12188200559040133No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT84780.12111060049172791No Hit
TGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAA82340.11762499226809242No Hit
GATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGGG81570.11652502573850253No Hit
TTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAA79100.11299656167605186No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG74030.1057539249162847No Hit
TTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAG73810.10543964876497329No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA73580.10511108733405683No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC72490.10355399185710491No Hit
GCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATA70180.10025409226833526No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)