FastQCFastQC Report
Sun 26 Apr 2020
403_S30_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename403_S30_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6761607
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT116520.17232589826649197No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG94260.13940473026604475No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT89810.1328234545426849No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA86960.12860848020300497No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA71410.10561098863036554No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT70060.10361442183788556No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC68580.10142559305798163No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)