FastQCFastQC Report
Sun 26 Apr 2020
401_S10_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename401_S10_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4142817
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG116260.28063030541778694No Hit
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA96590.23315053501035649No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG95860.2313884489708331No Hit
TCACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT93430.2255228748940636No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT91080.21985040613669393No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT76640.18499489598502664No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT72060.17393961644938696No Hit
GATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGGG67980.1640912451599962No Hit
CACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTG61520.14849799061846083No Hit
CCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAA54520.13160127517097667No Hit
ACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATC52430.1265563987016564No Hit
TACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCT43320.10456653045500199No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)