FastQCFastQC Report
Sun 26 Apr 2020
37_S70_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename37_S70_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7605760
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA824851.0845070052170986No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG636610.8370103710871761No Hit
TCAATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCG481830.6335067107034669No Hit
CAATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGG473270.6222520826321104No Hit
CGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGT319720.4203656176371592No Hit
TCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGG276110.3630274949511949No Hit
GGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGACTAAGCCATGCA213990.2813525538539212No Hit
TGTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCC202950.266837239145069No Hit
GCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTA161240.21199722315718614No Hit
CAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG141630.18621413244698756No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATGGCGATCTCGTATGCCGT138210.18171754039044094TruSeq Adapter, Index 15 (97% over 35bp)
CGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGGGGGGAGTA135270.17785204897340962No Hit
CGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGT133670.17574838017502523No Hit
CCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAA125120.1645069000336587No Hit
CAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTAC121620.15990512453719288No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGGCGATCTCGTATGCCGTC118250.15547427213059578TruSeq Adapter, Index 15 (97% over 34bp)
GCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAA117950.1550798342308987No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA114180.15012306462470548No Hit
AATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGGG113920.14978121844496803No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC109730.14427223577919893No Hit
TGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGGGGG107700.14160320599124873No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA106760.1403673005721979No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG105610.13885528862335914No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT104060.13681735947492427No Hit
GACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGGGGGG103090.13554201026590373No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA102240.13442443621676203No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA98680.12974377314035676No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT96850.12733770195220465No Hit
AGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGA96530.12691696819252776No Hit
ATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGGGG96110.12636475513295187No Hit
TTCAATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCC92150.12115817485695052No Hit
TAAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAA92050.1210266955570515No Hit
TCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGACC88010.11571493184113094No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG84420.11099482497475598No Hit
GCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAG81930.10772099040727028No Hit
AGCAAGCCAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGT81030.10653767670817907No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT80480.10581454055873442No Hit
ACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGGGGGGA76780.10094980646247055No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT76100.10005574722315717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)