FastQCFastQC Report
Sun 26 Apr 2020
35_S72_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename35_S72_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727730
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCGT453881.663947678105971TruSeq Adapter, Index 13 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCGCGTATGCCGT174320.6390661832366107TruSeq Adapter, Index 13 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCGTC132960.4874382728495856TruSeq Adapter, Index 13 (96% over 33bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCG132010.4839555234572337TruSeq Adapter, Index 13 (97% over 35bp)
TGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGAGCACACGTCTGA67000.24562548346060645No Hit
GGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGAGCACACGTCTGAA65440.23990644235316544Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGC62420.22883496533747844TruSeq Adapter, Index 13 (97% over 37bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCGCGTATGCCG53320.19547389221073933TruSeq Adapter, Index 13 (97% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCGCGTATGCCGTC52050.19081800618096367TruSeq Adapter, Index 13 (96% over 33bp)
CGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGGGGGGAGTA48900.1792699424063232No Hit
GCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCG43700.16020647204818658TruSeq Adapter, Index 22 (97% over 34bp)
TTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGAGCACACGTCTGAACT43550.15965656424939417Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
TATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGAGCACACGTCT43390.1590699959306823No Hit
TGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCGT42530.15591719121760583TruSeq Adapter, Index 22 (96% over 33bp)
CAAAGCTGAAACTTAAAGGAATTGACGGAAGAGCACACGTCTGAACTCCA42330.15518398081921597Illumina Multiplexing Index Sequencing Primer (96% over 26bp)
GTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGAGCACACGTCTGAAC42250.15489069665986002Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
ACGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCG39650.14535896148079172TruSeq Adapter, Index 22 (97% over 34bp)
GAGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCG36300.1330776873077614TruSeq Adapter, Index 13 (96% over 33bp)
GGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCGT35710.13091471663251128TruSeq Adapter, Index 22 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATG34990.12827515919830776TruSeq Adapter, Index 13 (97% over 37bp)
AGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCGT34470.12636881216249407TruSeq Adapter, Index 22 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCC34200.12537897812466775TruSeq Adapter, Index 13 (97% over 36bp)
TTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAA32070.11757028738181566No Hit
CCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGA31680.1161405271049554No Hit
GGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG31510.11551729826632401No Hit
ATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGAGCACACGTCTG31410.11515069306712908No Hit
CCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCG30160.1105681280771924TruSeq Adapter, Index 22 (97% over 34bp)
TGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGGGGG30080.11027484391783644No Hit
TTGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATCTCGTATGCCG29110.10671877348564557TruSeq Adapter, Index 22 (97% over 34bp)
TGCAAAGCTGAAACTTAAAGGAATTGACGGAAGAGCACACGTCTGAACTC28020.10272277681442078Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGGGGGGAG27450.10063312717900966No Hit
GGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGG27390.10041316405949269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)