FastQCFastQC Report
Sun 26 Apr 2020
34_S68_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename34_S68_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6927864
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA177800.25664476092486804No Hit
TCAATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCG169410.244534246053329No Hit
CAATGACTCGGCGAGCAGCCCCCCGGGAAACCAAAGTTTTTGGGTTCCGG152210.21970696884349925No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG138390.1997585402946709No Hit
GGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGACTAAGCCATGCA128590.1856127660704656No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT109330.1578119893808539No Hit
TCACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT103630.14958434518922428No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT79360.11455190228907497No Hit
TCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGG78430.11320949718412487No Hit
TGGATCCTCGCCAGACGGCCTACAGCGAAAGCAATTGCCAAGCATGTTTT77620.1120403056411038No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGACTAAGCC72830.1051261976274361No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT70140.10124332694752669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)