FastQCFastQC Report
Sun 26 Apr 2020
344_S27_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename344_S27_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11069045
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG303170.2738899335940906No Hit
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA280090.2530389929754554No Hit
CAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAAGGGCAGG252850.22842982389176303No Hit
CCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAA193100.1744504607217696No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT160980.14543260055406768No Hit
CACACACACGCACGGATCGCTATTTACCGCATTGCGGAGCATCCCGTAAC159780.1443484961891473No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA147630.13337193949432855No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA144460.13050809713033057No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA133540.1206427474095552No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA130250.1176704946090652No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG120050.10845560750724205No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT118840.10736246893928067No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)