FastQCFastQC Report
Sun 26 Apr 2020
343_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename343_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7922295
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTTAAATCTCGTATGCCGT356180.4495919427388149TruSeq Adapter, Index 8 (97% over 34bp)
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG214250.27043931083101547No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTCTTAAATCTCGTATGCCGTC168190.2122995924791995TruSeq Adapter, Index 8 (96% over 33bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTAAATCTCGTATGCCG149430.18861958561250244TruSeq Adapter, Index 8 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTTAAATCGCGTATGCCGT123370.15572507713989442TruSeq Adapter, Index 8 (97% over 34bp)
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA117410.14820200459589045No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA106120.13395108361907757No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT103570.13073231935947854No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG89080.11244216480199236No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT84670.10687559602362698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)