FastQCFastQC Report
Sun 26 Apr 2020
332_S48_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename332_S48_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5594209
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT117420.209895625994667No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT113660.20317438980202562No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT91210.16304360455606862No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA89480.15995112088232671No Hit
ACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATC77470.1384824914478526No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT73440.13127861329456944No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG73080.13063509068038037No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA70220.1255226610232117No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT61800.1104713821024563No Hit
CATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAG60920.10889832682332748No Hit
GCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATA59230.10587734566227325No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA57030.10194470746445118No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)