FastQCFastQC Report
Sun 26 Apr 2020
331_S53_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename331_S53_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6864652
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA157890.23000437604120352No Hit
TCACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTT146360.21320818593571822No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT142990.20829897859352522No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT125520.18284976427064328No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT124690.18164067166114173No Hit
CCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAA103570.1508743633326205No Hit
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG103230.15037907238415No Hit
CACTGCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTG98790.1439111552923586No Hit
ACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATC97050.14137643102665656No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG92640.13495221607737726No Hit
GCCTGTCCTGACCCAACCTCTCGGTTGTAAACCCTTGGTGCTCTTGACTG72920.10622534106608755No Hit
TACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCT72310.10533673083500808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)