FastQCFastQC Report
Sun 26 Apr 2020
319_S52_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename319_S52_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7954995
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT200960.25262115186747447No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT167880.21103721623960794No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT152820.19210571471132287No Hit
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG148490.18666259375398728No Hit
ACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATC126230.15868017516038666No Hit
GTGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTG105770.1329604858331149No Hit
GATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGGG93180.11713395168695895No Hit
TACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCT92530.11631685500745129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)