FastQCFastQC Report
Sun 26 Apr 2020
299_S21_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename299_S21_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9795350
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTA150620.15376683834676658No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG125090.12770345112732062No Hit
CCGACCTCAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAA101510.10363080441229766No Hit
CAGATCAGGCGAGACTACGCCCTGAACTTAAGCATATCACTAAGGGCAGG99110.10118066225300781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)