FastQCFastQC Report
Sun 26 Apr 2020
185_S60_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename185_S60_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156823
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT48410.22445049964693442No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG40490.1877298229850108No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA39460.18295428043933137No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT38300.17757599951410014No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA35710.16556759641379937No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA33940.15736108155374826No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT33080.1533737353505596No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC33030.15314191289688583No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA28190.13070149938126588No Hit
CCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCT27790.1288469197518758No Hit
TGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAA27460.127316891557629No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT27210.12615777928926017No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG25310.1173485260496573No Hit
CATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAG24730.11465938558704168No Hit
TTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAG24460.11340754433720338No Hit
TTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAA23740.11006930100430122No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA23440.10867836628225867No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA22610.10483011355127425No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT22540.10450556211613099No Hit
GCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATA22530.10445919762539625No Hit
TACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCT21930.10167732818131112No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)