FastQCFastQC Report
Sun 26 Apr 2020
181_S69_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename181_S69_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2730129
Sequences flagged as poor quality0
Sequence length100
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT98000.3589573972511922No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG50000.18314152920979193No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA42170.15446156573553851No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG37960.13904104897607403No Hit
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG37070.1357811297561397No Hit
AAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGA36540.13383982954651594No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA35350.12948106115132288No Hit
AAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTG34990.12816244214101238No Hit
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA34710.12713684957743754No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA34080.12482926630939417No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA34040.12468275308602633No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)