FastQCFastQC Report
Sun 26 Apr 2020
162_S62_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename162_S62_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6282792
Sequences flagged as poor quality0
Sequence length100
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG146800.23365408245251476No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT108540.17275758930106233No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT107440.1710067753317315No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA105490.16790305965882685No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA92460.1471638723675716No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT89140.14187959747831858No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA84150.1339372686538087No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA81850.13027647580884422No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA81790.13018097686506255No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA81380.12952840074922103No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC79430.12642468507631638No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG69530.11066735935233889No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT67140.10686331809170191No Hit
TGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAA66540.1059083286538851No Hit
CATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAG64180.10215203686513893No Hit
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA63840.1016108761837094No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT63620.10126071338984324No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)