FastQCFastQC Report
Sun 26 Apr 2020
156_S66_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename156_S66_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5179056
Sequences flagged as poor quality0
Sequence length100
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAG137190.264893833934215No Hit
GTTCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCA129670.25037381329724956No Hit
CAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGT125010.24137603455147036No Hit
GGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGGA116950.22581335285812706No Hit
CCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTG114400.2208896756474539No Hit
AGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAGG102330.197584270183601No Hit
AAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACA95920.18520749727363442No Hit
CGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTTT82320.15894788548337768No Hit
TTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGA79620.15373458020148845No Hit
AAAAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAAC79160.15284638744975917No Hit
CGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAGATTTAA72380.13975519863079294No Hit
ACGTGATTTGAGTTCAGACCGGCGCAAGCCAGGTCGGTTTCTATCTTCTT72100.13921455956452294No Hit
CGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATTA70570.13626035323811908No Hit
TGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAA66790.12896172584347418No Hit
AAAATCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAG64380.12430836816593603No Hit
GGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAAT62790.12123831061104573No Hit
TTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAA60840.11747314568523684No Hit
TCGACCTTTAATACCCTACGTGATTTGAGTTCAGACCGGCGCAAGCCAGG59420.11473133327772475No Hit
TGGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAA58450.11285840508386084No Hit
CATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAGAAATAG56960.10998143290978123No Hit
GGTCTTATGGTAAGTTTTCATGAACAGGATTAGTAGTAACTGTGAAAAAA56510.10911254869613304No Hit
TGATTCTGGGTTTAACTATAGCAACATTTTTATTAATTTCAATACTGTGG56210.10853329255370091No Hit
TCGCTTAATCTCCAAAAATGGTCTTATGGTAAGTTTTCATGAACAGGATT55280.10673759851216129No Hit
CAGGTCGGTTTCTATCTTCTTTTATAATATTCTTTTGGCATGTACGAAAG55070.1063321192124588No Hit
TTTTGGCATGTACGAAAGGACCGTTAAAAGAGGAAGTTTCCTTTTAAAAG53020.10237386890583922No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)