[2020-04-26 04:23:29.633] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored. [2020-04-26 04:23:29.633] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65 [2020-04-26 04:23:29.633] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.35. [2020-04-26 04:23:29.633] [jointLog] [info] parsing read library format [2020-04-26 04:23:29.633] [jointLog] [info] There is 1 library. [2020-04-26 04:23:29.686] [jointLog] [info] Loading pufferfish index [2020-04-26 04:23:29.686] [jointLog] [info] Loading dense pufferfish index. [2020-04-26 04:23:29.816] [jointLog] [info] done [2020-04-26 04:23:29.816] [jointLog] [info] Index contained 40,435 targets [2020-04-26 04:23:29.827] [jointLog] [info] Number of decoys : 0 [2020-04-26 04:23:30.968] [jointLog] [info] Computed 8,752 rich equivalence classes for further processing [2020-04-26 04:23:30.968] [jointLog] [info] Counted 91,344 total reads in the equivalence classes [2020-04-26 04:23:30.988] [jointLog] [info] Number of mappings discarded because of alignment score : 98,787 [2020-04-26 04:23:30.988] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 9,280 [2020-04-26 04:23:30.988] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0 [2020-04-26 04:23:30.988] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings to valid targets : 518 [2020-04-26 04:23:30.991] [jointLog] [warning] Only 91344 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2020-04-26 04:23:30.991] [jointLog] [info] Mapping rate = 89.7518% [2020-04-26 04:23:30.991] [jointLog] [info] finished quantifyLibrary() [2020-04-26 04:23:30.993] [jointLog] [info] Starting optimizer [2020-04-26 04:23:31.006] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2020-04-26 04:23:31.008] [jointLog] [info] iteration = 0 | max rel diff. = 223.417 [2020-04-26 04:23:31.099] [jointLog] [info] iteration = 100 | max rel diff. = 0.173575 [2020-04-26 04:23:31.156] [jointLog] [info] iteration = 167 | max rel diff. = 0.0070097 [2020-04-26 04:23:31.157] [jointLog] [info] Finished optimizer [2020-04-26 04:23:31.157] [jointLog] [info] writing output [2020-04-26 04:23:30.968] [fileLog] [info] At end of round 0 ================== Observed 101774 total fragments (101774 in most recent round)