[2020-04-26 03:31:52.547] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored. [2020-04-26 03:31:52.547] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65 [2020-04-26 03:31:52.547] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.35. [2020-04-26 03:31:52.547] [jointLog] [info] parsing read library format [2020-04-26 03:31:52.547] [jointLog] [info] There is 1 library. [2020-04-26 03:31:52.609] [jointLog] [info] Loading pufferfish index [2020-04-26 03:31:52.609] [jointLog] [info] Loading dense pufferfish index. [2020-04-26 03:31:52.726] [jointLog] [info] done [2020-04-26 03:31:52.726] [jointLog] [info] Index contained 40,435 targets [2020-04-26 03:31:52.740] [jointLog] [info] Number of decoys : 0 [2020-04-26 03:31:53.556] [fileLog] [info] At end of round 0 ================== Observed 56697 total fragments (56697 in most recent round) [2020-04-26 03:31:53.555] [jointLog] [info] Computed 8,621 rich equivalence classes for further processing [2020-04-26 03:31:53.556] [jointLog] [info] Counted 49,503 total reads in the equivalence classes [2020-04-26 03:31:53.590] [jointLog] [info] Number of mappings discarded because of alignment score : 49,151 [2020-04-26 03:31:53.590] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 4,787 [2020-04-26 03:31:53.590] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0 [2020-04-26 03:31:53.590] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings to valid targets : 268 [2020-04-26 03:31:53.593] [jointLog] [warning] Only 49503 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2020-04-26 03:31:53.593] [jointLog] [info] Mapping rate = 87.3115% [2020-04-26 03:31:53.593] [jointLog] [info] finished quantifyLibrary() [2020-04-26 03:31:53.595] [jointLog] [info] Starting optimizer [2020-04-26 03:31:53.606] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2020-04-26 03:31:53.610] [jointLog] [info] iteration = 0 | max rel diff. = 119.489 [2020-04-26 03:31:53.683] [jointLog] [info] iteration = 100 | max rel diff. = 0.230932 [2020-04-26 03:31:53.749] [jointLog] [info] iteration = 200 | max rel diff. = 0.330031 [2020-04-26 03:31:53.818] [jointLog] [info] iteration = 300 | max rel diff. = 0.0206814 [2020-04-26 03:31:53.841] [jointLog] [info] iteration = 336 | max rel diff. = 0.00983168 [2020-04-26 03:31:53.842] [jointLog] [info] Finished optimizer [2020-04-26 03:31:53.842] [jointLog] [info] writing output