FastQCFastQC Report
Tue 14 Apr 2020
380825_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380825_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10271364
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAAGGATATCTCGT149240.14529715819632136TruSeq Adapter, Index 9 (97% over 38bp)
CTCGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAG140600.13688542242296153No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAGGCTTGGTA116650.11356816874565052No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG109560.10666548279274302No Hit
CTGGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAG103800.10105765894383646No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)