FastQCFastQC Report
Tue 14 Apr 2020
380824_S5_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380824_S5_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9924736
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACCGAACATCTCGT252360.2542737660729716TruSeq Adapter, Index 6 (97% over 37bp)
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG148590.14971682873982745No Hit
CTCGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAG131450.1324468479564595No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA131240.13223525542644157No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG120050.1209603963269149No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACCGAACATCGCGT115630.11650687736177567TruSeq Adapter, Index 6 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)