FastQCFastQC Report
Tue 14 Apr 2020
380823_S4_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380823_S4_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8951023
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC460880.5148908677812581No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG292520.3268006349665284No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT229310.2561830083555813No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC169590.1894643774236755No Hit
CGCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGC168240.18795616992605202No Hit
CATGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGC160740.1795772393836995No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC149890.16745571986576283No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG143680.16051796537669494No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG116670.130342643516836No Hit
TGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGG101940.11388642393165564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100200.11194251204582983No Hit
CTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAAC98890.1104789921777656No Hit
CACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAAT95670.10688163799824893No Hit
CTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCT90630.10125099667378801No Hit
CAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCGAGAATG90370.10096052708165314No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG89570.10006677449046886No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)