FastQCFastQC Report
Tue 14 Apr 2020
380823_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380823_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8929344
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAACCAACTATCTCGT650710.728732144265021TruSeq Adapter, Index 5 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAACCAACTATCGCGT324540.3634533511084353TruSeq Adapter, Index 5 (97% over 36bp)
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG150530.16857901319514626No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA146620.1642001920857792No Hit
CTCGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAG136480.1528443746819475No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG119350.13366043462991234No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAGGCCTGTTC98800.11064642598605227No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT96890.10850741107073487No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAGGCTTGGTA90590.1014520215594785No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)