FastQCFastQC Report
Tue 14 Apr 2020
380822_S3_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380822_S3_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9453032
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGACTAGATCTCGT404480.42788387895016117TruSeq Adapter, Index 8 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGACTAGATCGCGT145200.1536015111342054TruSeq Adapter, Index 8 (97% over 41bp)
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG142770.151030907332166No Hit
CACCAGTACTTGCAGTCTCGCGGTTGCGGCCATAAGCTTGCCTCTGTCAC130400.13794515875964453No Hit
CTCGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAG126680.1340099134330657No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA123250.13038144798409654No Hit
CTCGCGGTTGCGGCCATAAGCTTGCCTCTGTCACTGCCACCATTGCAACC114290.12090300762760563No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG108430.11470393837659705No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAGGCCTGTTC108010.11425963648488653No Hit
GTTCCGATCCAGCAGTTCTTTGTGTTGTCTAATAATATTCAGCAGTCTTG102510.10844139742677271No Hit
GCCTCTGTCACTGCCACCATTGCAACCACAGCCATGATGACTGCTGCACT101990.10789130937036921No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAGGCTTGGTA97840.10350118353561058No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)