FastQCFastQC Report
Tue 14 Apr 2020
380822_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380822_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9491395
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGACTAGATCTCGT406240.42800873844150417TruSeq Adapter, Index 8 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGACTAGATCGCGT146530.15438194280187476TruSeq Adapter, Index 8 (97% over 41bp)
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG141720.14931419459415607No Hit
CACCAGTACTTGCAGTCTCGCGGTTGCGGCCATAAGCTTGCCTCTGTCAC131100.1381251122727481No Hit
CTCGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAG128070.13493274697765714No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA119620.12602994607220538No Hit
CTCGCGGTTGCGGCCATAAGCTTGCCTCTGTCACTGCCACCATTGCAACC115490.12167863628054675No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG108870.11470389758302126No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAGGCCTGTTC107050.11278637123415473No Hit
GCCTCTGTCACTGCCACCATTGCAACCACAGCCATGATGACTGCTGCACT101960.10742361897276428No Hit
GTTCCGATCCAGCAGTTCTTTGTGTTGTCTAATAATATTCAGCAGTCTTG100860.10626467447619661No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAGGCTTGGTA95990.10113371111411969No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)