FastQCFastQC Report
Tue 14 Apr 2020
380821_S2_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380821_S2_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8837133
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACACAACGAATCTCGT286110.32375884803363264TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACACAACGAATCTCGG203030.22974645736349109TruSeq Adapter, Index 1 (97% over 38bp)
CTCGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAG137540.15563871223846013No Hit
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG132430.14985629389079014No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA116760.1321242986837473No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACACAACGAATCGCGT114980.13011007076616365TruSeq Adapter, Index 1 (97% over 38bp)
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG104700.11847733874775902No Hit
CTGGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAG96010.1086438327905668No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAGGCCTGTTC91600.10365352654531737No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAGGCTTGGTA89520.10129982201241058No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)