FastQCFastQC Report
Tue 14 Apr 2020
380821_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380821_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8846443
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACACAACGAATCTCGT308010.3481738366482438TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACACAACGAATCTCGG191190.21612076175701353TruSeq Adapter, Index 1 (97% over 38bp)
CTCGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAG137980.15597229304478646No Hit
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG129960.14690650242136866No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACACAACGAATCGCGT122760.13876763802129286TruSeq Adapter, Index 1 (97% over 38bp)
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA118230.13364693583624515No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG103620.11713182349109127No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAGGCCTGTTC94050.1063139162259905No Hit
CTGGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAG93970.106223484399323No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAGGCTTGGTA89020.10062801512427084No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)