FastQCFastQC Report
Tue 14 Apr 2020
380820_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380820_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9322712
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC483740.5188833463910502No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG306810.3290995152483526No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT239680.25709257134619196No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG213120.22860300736523875No Hit
CTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAAC172080.18458148229828403No Hit
CACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAAT171350.1837984483485063No Hit
CGCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGC165950.17800614241864385No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC163100.17494909206677198No Hit
CATGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGC158940.17048687120228534No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA148720.15952439590539746No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC145250.15580230302083772No Hit
TGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGG145250.15580230302083772No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG133730.14344538370379778No Hit
GTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACAT112490.1206623137130054No Hit
GAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGAAGG112410.12057650177330374No Hit
CTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCT99330.10654624963208131No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)