FastQCFastQC Report
Tue 14 Apr 2020
380824_S5_L002_R2_001.fastp-trim.202004145558.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380824_S5_L002_R2_001.fastp-trim.202004145558.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9824608
Sequences flagged as poor quality0
Sequence length15-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC501340.5102900797670502No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG319270.3249697087150958No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT252680.2571909230373364No Hit
CGCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGC198950.20250171813470827No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC187760.19111195072617657No Hit
CATGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGC185010.18831285685902177No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC169830.17286185871232723No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG127950.13023420374634795No Hit
CTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAAC122030.12420851803960015No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG118410.12052389265810912No Hit
CACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAAT105880.1077702031470365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)