FastQCFastQC Report
Tue 14 Apr 2020
380823_S4_L001_R2_001.fastp-trim.202004144852.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380823_S4_L001_R2_001.fastp-trim.202004144852.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8760917
Sequences flagged as poor quality0
Sequence length15-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC442500.50508411391182No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG283590.3236989917836226No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT222710.2542085491735625No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC165400.18879302246557067No Hit
CATGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGC160240.18290322805249723No Hit
CGCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGC158900.1813737077979394No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC143730.16405816879671387No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG137680.15715249899068784No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG113100.12909607521678382No Hit
TGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGG98490.11241973871000033No Hit
CTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAAC95530.10904109695366365No Hit
CACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAAT91360.10428132123612174No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG88560.10108530876391135No Hit
CTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCT87920.10035479162740613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)