WARNING An augustus species is mentioned in the config file, dataset default species (fly) will be ignored INFO ****************** Start a BUSCO 3.0.2 analysis, current time: 04/08/2020 09:38:12 ****************** INFO Configuration loaded from /gscratch/scrubbed/samwhite/outputs/20200331_hemat_busco_megan/config.ini INFO Init tools... INFO Check dependencies... INFO Check input file... INFO To reproduce this run: python /gscratch/srlab/programs/busco-v3/scripts/run_BUSCO.py -i /gscratch/srlab/sam/data/Hematodinium/transcriptomes/20200408.hemat.megan.Trinity.fasta -o 20200408.hemat.megan -l /gscratch/srlab/sam/data/databases/BUSCO/metazoa_odb9/ -m transcriptome -c 28 --long -z INFO Mode is: transcriptome INFO The lineage dataset is: metazoa_odb9 (eukaryota) INFO Temp directory is ./tmp/ INFO ****** Step 1/2, current time: 04/08/2020 09:38:13 ****** INFO Create blast database... INFO [makeblastdb] Building a new DB, current time: 04/08/2020 09:38:13 INFO [makeblastdb] New DB name: /gscratch/scrubbed/samwhite/outputs/20200331_hemat_busco_megan/tmp/20200408.hemat.megan_1354846646 INFO [makeblastdb] New DB title: /gscratch/srlab/sam/data/Hematodinium/transcriptomes/20200408.hemat.megan.Trinity.fasta INFO [makeblastdb] Sequence type: Nucleotide INFO [makeblastdb] Keep MBits: T INFO [makeblastdb] Maximum file size: 1000000000B INFO [makeblastdb] Adding sequences from FASTA; added 6348 sequences in 0.300245 seconds. INFO [makeblastdb] 1 of 1 task(s) completed at 04/08/2020 09:38:13 INFO Running tblastn, writing output to /gscratch/scrubbed/samwhite/outputs/20200331_hemat_busco_megan/run_20200408.hemat.megan/blast_output/tblastn_20200408.hemat.megan.tsv... INFO [tblastn] 1 of 1 task(s) completed at 04/08/2020 09:38:37 INFO ****** Step 2/2, current time: 04/08/2020 09:38:37 ****** INFO Maximum number of candidate transcript per BUSCO limited to: 3 INFO Getting coordinates for candidate transcripts... INFO Extracting candidate transcripts... INFO Translating candidate transcripts... INFO Running HMMER to confirm transcript orthology: INFO [hmmsearch] 156 of 776 task(s) completed at 04/08/2020 09:38:41 INFO [hmmsearch] 311 of 776 task(s) completed at 04/08/2020 09:38:41 INFO [hmmsearch] 776 of 776 task(s) completed at 04/08/2020 09:38:42 INFO Results: INFO C:25.6%[S:20.7%,D:4.9%],F:11.7%,M:62.7%,n:978 INFO 250 Complete BUSCOs (C) INFO 202 Complete and single-copy BUSCOs (S) INFO 48 Complete and duplicated BUSCOs (D) INFO 114 Fragmented BUSCOs (F) INFO 614 Missing BUSCOs (M) INFO 978 Total BUSCO groups searched INFO BUSCO analysis done with WARNING(s). Total running time: 31.658100128173828 seconds INFO Results written in /gscratch/scrubbed/samwhite/outputs/20200331_hemat_busco_megan/run_20200408.hemat.megan/