FastQCFastQC Report
Fri 20 Mar 2020
73_R1_001.fastp-trim.202003182229.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename73_R1_001.fastp-trim.202003182229.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25187159
Sequences flagged as poor quality0
Sequence length15-150
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT17297496.867582802808367No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT13122235.209888896163319No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT9403103.733291237808917Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT8556943.397342272703325No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT5933992.3559584469213064Illumina PCR Primer Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCT5902102.3432972333243303RNA PCR Primer, Index 12 (95% over 21bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT2541031.0088593159712853Illumina PCR Primer Index 10 (96% over 26bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTGCT2420510.9610095366452406TruSeq Adapter, Index 12 (95% over 24bp)
CACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTG1423860.5653118718153166RNA PCR Primer, Index 29 (95% over 23bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1284230.50987489299607No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG1269770.504133872343443Illumina PCR Primer Index 10 (95% over 24bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA1214050.4820114884731541No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG1206450.4789940778950099Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTGCTTG1201260.4769335040923035TruSeq Adapter, Index 12 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC1192820.47358259023973287No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTC1022520.40596877162684364No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT939500.37300753133769476No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCT831490.33012456863435846No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACACTCGCT548800.21788880595862362No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTGC515770.20477498077492584RNA PCR Primer, Index 29 (95% over 24bp)
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC512270.20338538379814888Illumina PCR Primer Index 10 (96% over 25bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA469830.1865355278854594No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT398040.1580329087532262No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA264890.10516866947955503Illumina PCR Primer Index 10 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)