FastQCFastQC Report
Fri 20 Mar 2020
485_R2_001.fastp-trim.202003181245.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename485_R2_001.fastp-trim.202003181245.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25310261
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG654210.25847619666980126No Hit
ATCACCATATTGGGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGC601740.23774547405892021No Hit
ATCACCATATTGGGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGC500930.1979157781107038No Hit
TCACCATATTGGGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCC412960.16315912348750572No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG389840.1540244883290615No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC375670.14842596842442676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359350.14197799066552494No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT357400.1412075521465385No Hit
AGTTTTCATCAAGAACAGGCCTGGATTTCAAGGAGTCCAGACCCTGCCTG342240.13521788653226452No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA340100.13437237964476148No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA331890.1311286359314904No Hit
AGTTTTCATCAAGAACAGGCCTGGATTTCATGGAGTCCAGACCCTGCCTG327330.12932699508709136No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT308560.12191103047100146No Hit
TTGTTAGCCCTTGTAAGTTGGACAGCACCTGCCCCAGCGCCTGCAGCAGA307410.12145666929313768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)