FastQCFastQC Report
Fri 20 Mar 2020
485_R1_001.fastp-trim.202003181245.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename485_R1_001.fastp-trim.202003181245.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25310261
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG621760.2456553095205142No Hit
ATCACCATATTGGGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGC607990.24021482828644083No Hit
ATCACCATATTGGGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGC494790.19548988451758756No Hit
TCACCATATTGGGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCC417970.16513855783628623No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC399750.15793989639221814No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT393690.1555456105332142No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG385010.15211617138203357No Hit
AGTTTTCATCAAGAACAGGCCTGGATTTCAAGGAGTCCAGACCCTGCCTG348290.13760822142450446No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA345270.1364150294617665No Hit
AGTTTTCATCAAGAACAGGCCTGGATTTCATGGAGTCCAGACCCTGCCTG343940.13588955088215013No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT330430.13055179478394158No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA324860.1283511062963752No Hit
TTGTTAGCCCTTGTAAGTTGGACAGCACCTGCCCCAGCGCCTGCAGCAGA301240.11901892279972932No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296780.11725679162297063No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG257430.10170973740650086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)