FastQCFastQC Report
Fri 20 Mar 2020
463_R2_001.fastp-trim.202003185732.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename463_R2_001.fastp-trim.202003185732.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19061045
Sequences flagged as poor quality0
Sequence length15-150
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG750243139.360019348362066No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA644140.33793530207813893No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT577610.30303165435053536No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA290100.15219522329442062No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT275180.14436774059344595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)