FastQCFastQC Report
Fri 20 Mar 2020
463_R1_001.fastp-trim.202003185732.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename463_R1_001.fastp-trim.202003185732.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19061045
Sequences flagged as poor quality0
Sequence length15-150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT15968598.377604690613762No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT10818415.675664686799701No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT8573224.497770190459127TruSeq Adapter, Index 12 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT6840143.5885440698555615No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT5823013.054926946555134RNA PCR Primer, Index 12 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT4930462.586668254547429Illumina PCR Primer Index 12 (95% over 22bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT2569291.3479271467015579TruSeq Adapter, Index 12 (96% over 27bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGCT2072941.0875269430401113Illumina PCR Primer Index 12 (96% over 25bp)
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG1230900.6457673228304115TruSeq Adapter, Index 12 (96% over 25bp)
CACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTG1197970.6284912500862361Illumina PCR Primer Index 12 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC1084590.5690086771213225No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT845040.44333351083322037RNA PCR Primer, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTC843540.4425465655214601No Hit
GCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG697280.36581415132276324No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG667380.3501277081083435TruSeq Adapter, Index 12 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCT659960.3462349519661697No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA659480.34598312946640647No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT630330.3306901589078668No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGCTTG535380.2808765206734468Illumina PCR Primer Index 12 (96% over 27bp)
GCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCG516760.27110790620346364No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCGTAGT506120.2655258407920447No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC499330.26196360168081023Illumina PCR Primer Index 5 (96% over 25bp)
ACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGC430670.2259424916105072Illumina PCR Primer Index 12 (95% over 24bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA310270.16277701458655597No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT272120.14276237215745516No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCG269610.14144555033577647Illumina PCR Primer Index 12 (95% over 21bp)
GATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT257550.1351185100292245TruSeq Adapter, Index 12 (95% over 21bp)
GATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCT193050.10127986162353639No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCG192590.10103853172792991RNA PCR Primer, Index 12 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)