FastQCFastQC Report
Fri 20 Mar 2020
445_R2_001.fastp-trim.202003185018.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename445_R2_001.fastp-trim.202003185018.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29847696
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA8527682.8570647463040366No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT7491382.509868768430233No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA599200.20075251369485939No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG544860.18254675335744508No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT536260.17966545893525585No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA461730.15469535739039958No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT458620.1536534009191195No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT454800.15237357014089128No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT451990.15143212394015268No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT440690.1476462370830901No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT435120.14578009639336986No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT425040.14240295130317598No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT420150.14076463389334976No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT409930.1373405840102365No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG378130.1266864953328391No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA377160.12636151212475497No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT362150.12133264825532931No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT361380.12107467189427285No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT345950.11590509364608913No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT307110.1028923639533182No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT302720.10142156366106114No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT299420.10031595068510481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)