FastQCFastQC Report
Fri 20 Mar 2020
427_R2_001.fastp-trim.202003184618.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename427_R2_001.fastp-trim.202003184618.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18572313
Sequences flagged as poor quality0
Sequence length15-150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257210613.849141999706768No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1323220.7124691469500865No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT1206650.6497036744965476No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA949310.5111425808944745No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA752550.40519993390160935No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT197280.10622263365903858No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT191810.10327738930525239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)