FastQCFastQC Report
Fri 20 Mar 2020
427_R1_001.fastp-trim.202003184618.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename427_R1_001.fastp-trim.202003184618.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18572313
Sequences flagged as poor quality0
Sequence length15-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT4893572.634873749973953No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT3153791.6981137459830664No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT2065281.1120208882975426TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT1819270.979560273402672RNA PCR Primer, Index 12 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT1561180.8405953528782333Illumina PCR Primer Index 12 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT1453010.7823527419551888No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1357120.73072212384101No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT1192950.64232710271467TruSeq Adapter, Index 12 (96% over 27bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1057940.5696328723299031No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGCT977500.5263210888164549Illumina PCR Primer Index 12 (96% over 25bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT681680.36704098191754575No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG572860.3084483876617845TruSeq Adapter, Index 12 (96% over 25bp)
CACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTG554910.29878346331983524Illumina PCR Primer Index 12 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC470250.25319948032321016No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT373990.2013696409273309No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTC319010.17176643533845246No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG293010.15776710202977948TruSeq Adapter, Index 12 (96% over 29bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCGTAGT278600.15000824076139574No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT268980.14482848743718674RNA PCR Primer, Index 12 (95% over 21bp)
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC251750.13555123694070847Illumina PCR Primer Index 5 (96% over 25bp)
ACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGC222950.12004428312187071Illumina PCR Primer Index 12 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTTTGCCGT189880.10223820802503167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)