FastQCFastQC Report
Fri 20 Mar 2020
425_R2_001.fastp-trim.202003182722.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename425_R2_001.fastp-trim.202003182722.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62615266
Sequences flagged as poor quality0
Sequence length31-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA767233912.253144464801922No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT706509511.283342627658884No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA8121061.296977641203345No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT7507971.1990638193567684No Hit
CACACACACACACACACACACACACACACACACACACACCT2252010.3596582980259159No Hit
CACACACACACACACACACACACACACACACACACACACACCT2216150.353931260149881No Hit
CACACACACACACACACACACACACACACACACACACACACACCT2198340.3510869058673327No Hit
CACACACACACACACACACACACACACACACACACACCT2163390.345505199961939No Hit
CACACACACACACACACACACACACACACACACACACACACACACCT2158760.34476576367175377No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT2104110.33603786016017245No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCT2101180.33556992315580036No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT2045030.3266024614508545No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT2037770.3254429997949701No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1966220.3140160739714817No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1876370.2996665381889458No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1819760.29062561197136816No Hit
CACACACACACACACACACACACACACACACACACCT1795500.28675115745735236No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1745090.2787004051056814No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACCTGT1654860.26429018124749326No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1636500.2613579889607113No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1500300.23960610500321117No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1495370.2388187570743531No Hit
CACACACACACACACACACACACACACACACACCT1489890.2379435711412613No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1446930.2310826244833009No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1381910.22069857532825943No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1363270.21772166551204938No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1309520.20913749691648678No Hit
CACACACACACACACACACACACACACACACCT1307070.20874621853399136No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1275060.20363404668759214No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1236210.1974294894794506No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1221770.1951233426046613No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1127620.18008707333448043No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1112300.1776403856529173No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1095870.17501642490826438No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA1064890.17006874968797545No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT978430.15626061542244346No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT974560.15564255528356297No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT945120.15094082647512827No Hit
CACACACACACACACACACACACACACACCT926510.14796870782278557No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT892920.14260420134604235No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT860000.13734669752900197No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT775750.12389151233502704No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT771100.12314888193559698No Hit
GTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT707300.11295967344449194No Hit
CACATACACACACACACACACACACACACACACACACACACACACACACA704080.1124454218560694No Hit
CACCCACACACACACACACACACACACACACACACACACACACACACACA682140.10894148401445743No Hit
GTGTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT670390.10706494483310189No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT660610.10550302541236509No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT655020.10461027187842657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)