FastQCFastQC Report
Fri 20 Mar 2020
425_R1_001.fastp-trim.202003182722.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename425_R1_001.fastp-trim.202003182722.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62615266
Sequences flagged as poor quality0
Sequence length31-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA801490112.800234690370877No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT725540711.587281286962831No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA8062671.2876524392629747No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT7239231.1561445734335776No Hit
CACACACACACACACACACACACACACACACACACACACACCT2312100.3692549992520993No Hit
CACACACACACACACACACACACACACACACACACACACCT2311100.3690952937898563No Hit
CACACACACACACACACACACACACACACACACACACACACACCT2295140.36654639461245764No Hit
CACACACACACACACACACACACACACACACACACACACACACACCT2260450.36100621212724704No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCT2223530.35510988646123454No Hit
CACACACACACACACACACACACACACACACACACACCT2222330.3549182399065429No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT2184340.3488510293959304No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT2137990.34144868122096617No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT2111670.33724523345472973No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT2094360.33448073190330296No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT2044480.3265146234466208No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1980730.31633340022862794No Hit
CACACACACACACACACACACACACACACACACACCT1860000.2970521597720275No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1813280.28959072057603336No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1764160.28174598827065594No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1708520.272859976351454No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACCTGT1707190.27264756808667073No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1655350.2643684369239923No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1589270.25381509997897317No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1559820.24911177411591606No Hit
CACACACACACACACACACACACACACACACACCT1525130.24357159163070552No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1490520.2380441855824744No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1467700.23439970693408854No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1434620.22911665024308928No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1406310.22459538860698922No Hit
CACACACACACACACACACACACACACACACCT1340730.2141219043930916No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1298120.20731685464691632No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1290990.20617815470112352No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1278250.20414350711214735No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1173280.187379224740497No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1150150.18368523739881581No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1107750.1769137257997115No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA1088370.1738186339414417No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1059930.16927661059525007No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1059240.16916641382630235No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT947510.15132252252988912No Hit
CACACACACACACACACACACACACACACCT947070.1512522521265022No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT937010.14964561517633734No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT924880.14770838791932944No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA881500.140780364967227No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT871550.13919129561790888No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT825810.13188636777491292No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT779860.12454790178484589No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG735140.1174058735133378No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT734900.11736754420239945No Hit
GTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT730230.11662171969372453No Hit
CACCCACACACACACACACACACACACACACACACACACACACACACACA714220.11406483524321369No Hit
CACATACACACACACACACACACACACACACACACACACACACACACACA708120.11309063192353123No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT693220.11071102053611015No Hit
GTGTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT692380.11057686794782601No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGT689730.110153648472882No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC668310.1067327574716364No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT639360.10210928433970079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)