FastQCFastQC Report
Fri 20 Mar 2020
334_R2_001.fastp-trim.202003181149.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename334_R2_001.fastp-trim.202003181149.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25348734
Sequences flagged as poor quality0
Sequence length15-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA859090.3389084441061238No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA660230.2604587668954197No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG583930.23035864434097575No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG499100.19689346221393147No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG479630.18921260525279093No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG467570.1844549712029011No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT414460.163503234520509No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT413740.16321919666678422No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC404410.15953853947893415No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT394290.15554622964602494No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG379630.14976290334657344No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC363960.14358113505786918No Hit
CTTTAGAACTAACAACACAAACTCAAGAGAAAATAAGTGCTTCACAATTT333270.131474021542851No Hit
TTGTTAGCCCTTGTAAGTTGGACAGCACCTGCCCCAGCGCCTGCAGCAGA328640.12964750034459316No Hit
GTATATAAATGAATTGTCTACTGTGACATGGGGTTACAGATAGATTTCTT324500.12801428268567575No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA311740.1229805007224424No Hit
CAATTATTTATTGCAAATATTTTCACGTGTATGGAAAGTAAATTAACACT300080.11838066548017744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287530.11342972789094713No Hit
GTCCAAGTACCTACGGCGGGCGTGGCATCTGCTGCAGGCGCTGGGGCAGG261090.10299922670694323No Hit
CAATTAAGGAAGTCAATAGTCACCTGAATAGCAAACCAATGATGCAAGAT255950.10097151202896365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)