FastQCFastQC Report
Fri 20 Mar 2020
294_R2_001.fastp-trim.202003180701.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename294_R2_001.fastp-trim.202003180701.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28208357
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATAATATTCAGCAGTCTTGCATACGTGGTGCTTCAGGCAGACGTC536860.1903194858176249No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA532190.18866394806333456No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT513280.18196026092551226No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT495370.17561107866012898No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC494730.175384195541768No Hit
GTATATAAATGAATTGTCTACTGTGACATGGGGTTACAGATAGATTTCTT434050.15387283988216682No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA424350.1504341426195081No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA342780.12151718017465533No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT307970.10917686556505223No Hit
ATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCA301740.10696830020975698No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG299830.10629119590339842No Hit
GTTATACTGCTGTGGCAAAGCAGGAACAACATACCCTTCCTTCACTAGCG298000.10564245198683496No Hit
CTGTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTT292010.1035189678009251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)