FastQCFastQC Report
Fri 20 Mar 2020
294_R1_001.fastp-trim.202003180701.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename294_R1_001.fastp-trim.202003180701.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28208357
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT563570.19978831096047175No Hit
GTCTAATAATATTCAGCAGTCTTGCATACGTGGTGCTTCAGGCAGACGTC544400.1929924525558153No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA536010.1900181566760517No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT511500.18132924225257074No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC467300.16566012689076504No Hit
GTATATAAATGAATTGTCTACTGTGACATGGGGTTACAGATAGATTTCTT434940.15418834921863758No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA422670.14983857443381052No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT351070.12445602556717501No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA347570.12321525851363836No Hit
GTTATACTGCTGTGGCAAAGCAGGAACAACATACCCTTCCTTCACTAGCG297420.10543683916082033No Hit
CTGTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTT292960.1038557474297422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)