FastQCFastQC Report
Fri 20 Mar 2020
280_R2_001.fastp-trim.202003185124.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename280_R2_001.fastp-trim.202003185124.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58003573
Sequences flagged as poor quality0
Sequence length31-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA606461910.455595554432483No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT53667019.25236278116867No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA3109180.5360324957912507No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT3024870.5214971843200072No Hit
CACACACACACACACACACACACACACACACACACACACCT1267790.21857101803021686No Hit
CACACACACACACACACACACACACACACACACACACACACCT1266760.2183934427625691No Hit
CACACACACACACACACACACACACACACACACACACACACACCT1254800.21633150081978572No Hit
CACACACACACACACACACACACACACACACACACACACACACACCT1232560.2124972542639744No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCT1204630.2076820336567887No Hit
CACACACACACACACACACACACACACACACACACACCT1203180.20743204905670207No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1173000.20222892131145093No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1145160.19742921698978785No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1137180.19605343967344907No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1126200.19416045283968972No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1124660.1938949519540805No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1078500.18593682151270233No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1045840.18030613389971684No Hit
CACACACACACACACACACACACACACACACACACCT1012360.17453407568530305No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT970550.16732589904418474No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACCTGT962530.16594322560094704No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACCTGT953610.16440538930248316No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT887580.15302160782405594No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT861420.1485115408321484No Hit
CACACACACACACACACACACACACACACACACCT852990.14705818208819654No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT824800.1421981366561677No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT817530.14094476559228514No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT793640.13682605380189253No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT790330.1362553993010051No Hit
CACACACACACACACACACACACACACACACCT753070.1298316570946414No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT745400.1285093247617694No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT740930.12773868258081272No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT725290.12504229696332672No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT681490.1174910380089861No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT672910.11601181878916321No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA653120.11259996000591206No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT602520.10387635947875143No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT591200.10192475556635107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)