FastQCFastQC Report
Fri 20 Mar 2020
280_R1_001.fastp-trim.202003185124.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename280_R1_001.fastp-trim.202003185124.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58003573
Sequences flagged as poor quality0
Sequence length31-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA624860610.772794979371357No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT57158759.854349834621395No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA3245850.559594837373208No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2895860.49925545103919716No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1449520.24990184656383152No Hit
CACACACACACACACACACACACACACACACACACACACACACCT1339300.23089956889379898No Hit
CACACACACACACACACACACACACACACACACACACACACACACCT1326570.22870487650821095No Hit
CACACACACACACACACACACACACACACACACACACACACCT1325180.22846523609847277No Hit
CACACACACACACACACACACACACACACACACACACACCT1318350.22728772243047857No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCT1312900.2263481251404978No Hit
CACACACACACACACACACACACACACACACACACACCT1253970.21618840618663268No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1245640.21475228775992816No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1238560.2135316732988156No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1204460.2076527251174682No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1188710.20493737515101007No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1176570.20284440063718145No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1145880.19755334727396878No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1077230.18571786948366092No Hit
CACACACACACACACACACACACACACACACACACCT1050320.18107850011239826No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1035310.17849072849357056No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACCTGT1008690.17390135604232518No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1007270.17365654353741278No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT967260.16675869260674683No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT948870.1635881982649586No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT883410.15230268659484134No Hit
CACACACACACACACACACACACACACACACACCT876510.15111310470477396No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT871080.1501769554782427No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT849210.1464064980962466No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT822370.14177919694705704No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT815510.1405965111838886No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT795600.13716396401994063No Hit
CACACACACACACACACACACACACACACACCT773070.133279720544112No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT755910.13032128210446622No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT751530.12956615620903217No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT720690.12424924236994847No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA702430.12110116044058182No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT686240.11830995307823539No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT674900.11635490110238553No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA658840.11358610615246063No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC647420.11161726192281292No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT623720.10753130673519025No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT597540.10301779167983323No Hit

[OK]Adapter Content

Adapter graph